The protocol at a glance
No human dose exists. Mouse papers gave 5 mg per kg into the belly cavity every other day for three doses.
| Item | Finding |
|---|---|
| Human dose | Not established |
| Studied in | Mice (injection into the belly cavity) and cell cultures |
| Mouse schedule (aged mice 2020; lung fibrosis mice 2022) | 5 mg per kg, every other day, 3 doses |
Sources: Aging 2020 (PMID 31959736) and J Cell Mol Med 2022 (PMID 35510614), whose full texts give the 5 mg per kg dose. Mouse doses do not convert directly to people.
Community reports commonly describe either an escalating 250-500 mcg daily plan or 3 mg every other day for 3 doses.
What people say they do, collected from community discussions. Not tested in a trial; shown so you can compare it with the evidence above.
Mixing
No mixing table here: this one is used as a capsule, nasal spray or skin product, or has no human amount to calculate from. The general mixing calculator works for any vial.
What the trials found
There are no human results, so these are the evidence facts, all from mice and cells:
Baar MP et al. Cell 2017 (PMID 28340339): in aged and fast-aging mice, restored fitness, fur density and kidney function. Aging 2020 (PMID 31959736) and J Cell Mol Med 2022 (PMID 35510614) are later mouse studies.
Side effects
- Side effects in people are unknown.
- The 2017 paper says it was well tolerated in mice at the doses used. Because the peptide is built to kill cells, its effects on healthy tissue in people have not been tested.
How it works
FOXO4-p53 link
Aged cells use FOXO4 to hold p53 inside the nucleus and stay alive. FOXO4-DRI blocks that link, so p53 leaves the nucleus and those cells die by apoptosis.
Sources
- Baar MP et al. Cell 2017, PMID 28340339
- FOXO4-DRI alleviates age-related testosterone secretion insufficiency, Aging 2020, PMID 31959736
- FOXO4 peptide in bleomycin-induced pulmonary fibrosis in mice, J Cell Mol Med 2022, PMID 35510614
- Targeting senescence-like fibroblasts radiosensitizes NSCLC, JCI Insight 2021, PMID 34877934