5-Amino-1MQ quick reference
What is 5-Amino-1MQ?
Small molecule that blocks the NNMT enzyme.
Used for fat loss without cutting appetite.
Mice only. No human trial exists.
How 5-Amino-1MQ works
Blocks NNMT, an enzyme that uses up nicotinamide and SAM.
In fat cells it raised NAD+ and SAM and cut fat making.
Treated mice ate the same but lost fat.
What 5-Amino-1MQ is studied for
Obese mice lost weight and fat with the same food intake.
Obese mice had less liver fat and better liver enzymes.
Mice had better glucose tolerance and insulin response.
Old mice regrew injured muscle with larger fibers.
Cultured fat cells made less fat and held more NAD+.
5-Amino-1MQ dosing protocol
| Item | What is known |
|---|---|
| Human dose | Not established |
| Studied in | Mice (injection under the skin) |
| Mouse dose | 20 mg/kg per injection, 3 times a day, 11 days |
| Human trials | None found |
| Oral exposure | Measured in rats (2021) and mice (2024); no human data |
From Neelakantan H et al. Biochem Pharmacol 2018 and a 2022 Scientific Reports mouse study (32 mg/kg by injection). Do not convert mouse doses into human doses.
Label and trial amounts come from the sources below. Community amounts are what people report; they have not been tested.
Mixing
Injectable form only. Capsules need no mixing.
What the research found
No human results exist. In obese mice fed a high-fat diet:
Neelakantan H et al. Biochem Pharmacol 2018 (PubMed 29155147): treated mice lost weight and fat, ate the same food, and showed no visible side effects.
5-Amino-1MQ community experience
What users report. Not tested in trials.
Positive reports
- Modest fat loss About 3-6 lb over 6-8 weeks with diet and training.
- Appetite unchanged Fits an energy-use mechanism, not an appetite one.
- Mild warmth Some feel warmer or more energetic in week one.
Negative & mixed
- Little effect Weak results when diet and sleep are loose.
- Subtle at best Hard to tell from normal ups and downs.
- More is not better Injections of 5-10 mg are called excessive.
5-Amino-1MQ stacking
Aims to save NAD+ building blocks. Not studied together.
Different pathways. Not studied together.
Appetite plus energy use. Not studied together.
5-Amino-1MQ side effects & safety
Common
- No human safety data exists.
- Mild jitters or energy in the first days (community).
- Feeling warmer (community).
- Injection-site redness (community).
- Poor sleep if taken late (community).
Less common
- Headache (community).
- Nausea (community).
- Overstimulation in sensitive people (community).
Precautions
- Cancer history: NNMT has mixed roles in cancer; ask a doctor.
- Pregnancy or breastfeeding: never studied.
- Severe liver or kidney disease: clearance unknown.
- Anxiety or stimulant sensitivity: use care.
Our verdict on 5-Amino-1MQ
Interesting mouse data. Without a human trial, dose and benefit are guesses.
5-Amino-1MQ FAQ
Does 5-Amino-1MQ work in humans?
No human trial exists. Evidence is from mice and cells.
Is it a peptide?
No. It is a small molecule.
Does it work by mouth?
Oral levels were measured in rodents. Human absorption is unknown.
Is it an appetite suppressant?
No. Treated mice ate the same.
Sources
- Neelakantan H et al. Selective and membrane-permeable small molecule inhibitors of NNMT reverse high fat diet-induced obesity in mice. Biochem Pharmacol 2018 (PubMed 29155147)
- Reduced calorie diet combined with NNMT inhibition establishes a distinct microbiome in DIO mice. Sci Rep 2022 (PubMed 35013352)
- ClinicalTrials.gov search for 5-amino-1MQ, 5 Nov 2026: no registered studies
- Nicotinamide N-methyltransferase inhibition mitigates obesity-related metabolic dysfunction. Diabetes Obes Metab 2024 (PubMed 39161060)
- Neelakantan H et al. NNMT inhibitor activates senescent muscle stem cells in aged muscle. Biochem Pharmacol 2019 (PubMed 30753815)
- LC-MS/MS assay for 5-amino-1-methyl quinolinium in rat plasma, with pharmacokinetic and oral bioavailability studies. J Pharm Biomed Anal 2021 (PubMed 34304009)